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AccuBondODS2C18100mg,1mlOASISHLB1CC,30mg(SPE),OA2SIS2Rd,5000ng/ml,,,012m,10mmol/L=32,,012m,2Rd,,10800r/min,3min,5l,LC/MS/MSLC/MS/MS1032Rd812Rd8002Rd,(2006-02-13)DeterminationofGinsenosideRdinHumanPlasmabyLC/MS/MSDENGYuan2hui,FENGYi,ZENGXing,YANGLiu,LIUYi2ming,HUANGYu,SUNJing,ZHOUDan,LIANGWei2xiong(DepartmentofClinicalPharmacology,GuangdongProvincialHospitalofTraditionalChineseMedicine,Guangzhou510120,China)AbstractObjective:TodevelopaHPLC/MS/MSmethodforthedeterminationofginsenosideRdinhumanplasma1Methods:Plasmasampleswerepretreatedbysolidphaseextraction(SPE)1GinsenosideRd(m/z96416m/z76715)andgentiopicrin(m/z3741119511)wasdetectedbythepositiveelectrosprayionization(ESI+)2MSmethodundermultiplereactionmonitoring(MRM)mode1Results:ThecalibrationcurveofginsenosideRdinplasmawaslinearovertherangeof31005000100ng/ml1Thelimitofquantitationwas3100ng/ml1Therelativerecoverywas8110183139%1Thewithin2dayandbetween2dayRSDswerelessthan15%1Conclusion:Thismethodisaccurate,sensitive,andspecific1ItissuitableforthemeasurementofplasmaginsenosideRdconcentra2tion1KeywordsGinsenosideRd;LC/MS/MS;Plasmaconcentration,,,,,,3(,430072)::,K2BpH,,:,pH6,1ml100l2(21),50l:,,pH,;;;:R28515:A:100124454(2006)09209312063:,Tel:(027)87534950,Email:dinghong2000@2631net,(Bacterialvaginitis,BV)(Candidalvaginitis,CV)70%75%,40%50%190%2,,B3(RadixSophoraeFlavescentis)SophoraflavescensAit,104,,,(CortexPhellodendri)13929920069©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(FructusCnidii)Cnidiummon2nieri(L1)Cuss1,,,(ZanthoylumbungeanumMaxim)(ZanthoxylumachinifoliumSieb1etZucc1),,,,5,,,,,,,,1111(Echerichiacoli,HB101),(Staphylococcusaureus)(Pseudomonasaeruginosa),(C1albicans,AY93025)112(),(),(),(),(),(),(),(),113,114SD(68),(231),115,;,,;,;NaCl,;,OXOID;80,;,;,221121111:7,50g20260600ml60%2,2h,,5ml,10g/ml211128:3050g,300ml70%2,1h,,15ml,313g/ml212950g,,,1000ml,300500ml(),,,,5h,,2132131110,:50g,170ml015h,1h,,25ml,2g/ml;10ml,5g/ml21312:50g,830min,,6,30min,,20ml,215g/ml214(,),37()30(),108109cFu/ml215(K2B)11610mm,,6,3,20%80373018h,216415ml,,10%1%20%Tween801%011%,217,43120%8010%,215,373016h,218MIC23929920069©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(),1L9(34)AB(l)C(l)DpH111100100421250505313202062191220010gSD,(118109/ml)(118109/ml),01025ml/100g,521101368KM,215106/L20l(5104)52111:,,Tween80()6,,5,62%,10%()25,,;,,,,,,0,1,2,3,2,+,++,+++,33112,,,,,2a(mm)11190131211011171101111160141217012121001112130142015012601010180129133013915001460106010713301260108130121110011710001371500131118011111001312100146010121001281500151214012601060101215012121301212180111160109120012601060101111100111210013121401416014Tween6010601081500129155015a,S1E1M,2312MIC,3:,11103,3313,DABC,A2B2C2,1ml12100l,33929920069©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(mm)111111416321222111133133391781421231012552231161006231210167731329154283213912819332114175K1351541341422341581451380K23619236141136113311342K3331573351201351323291312R111160166301516513565FA1188620194306287170101B01669620133482232100101C01400120120011333170101D511042201501701400101F0105(2,2)=19;F0101(2,3)=3018314MICpH6,12179mm,4pH5pH6,10188mm,6MIC:MBC1%,0121mg/ml;MBC10%,211mg/ml6a(mm)81250131210012151801215110139100011121001210100111215012a,S1E1M,23157,,;8,,7n(%)(%)6161716176661733661780633133313650333,3P0105,33P01018n-++++++-++++++642004200363210222036015002408036114024006510060004,,1415,,,,,11,;,43929920069©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.[1]M1Inhof,M1Lipovac,Ch1Kurz1Propolissolutionforthetreatmentofchronicvaginitis1InternationalJournalofGy2necologyandObsterics,2005,89:12721321[2]ArsenioS,EzioC,ThomasO1E,etal1Torulopsisgla2bratavaginitis1ObstetricsandGynecology,1995,85:99329981[3]GilbertG1G1Donders,AnnieV,EugeneB,etal1Aero2bicvaginitis:Abnormalvaginalfloraentitythatisdistinctfrombacterialvaginosis1InternationalCongressSeries,2005,1279:11821291[4]YangFQ,QuanJ,ZhangTY,etal1Preparativesepara2tionofalkaloidsfromtherootofSophoraflavescensAitbypH2zone2refiningcounter2currentchromatography1JournalofChromatography,1998,822:31623201[5]LeeHS,YooEE,DongOE1Narrowborehighperform2anceliquidchromatographyofberberineandpalmatineincrudedrugsandpharmaceuticalswithion2pairextractionu2singcobaltthiocyanatereagent1JournalofPharmaceuticalandBiomedicalAnalysis,1999,21:592631[6]YasunoriN,SeijiH,X2YZhang1EffectsofTopicalInstil2lationofTraditionalHerbalMedicines,HerbalExtracts,andTheirComponentsonProstaglandinE22inducedAque2ousFlareElevationinPigmentedRabbits1JpnJOphthal2mol,2003,47:24922531[7]KongLM,Zhan
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本文标题：从中药筛选治疗阴道炎的抗菌组方
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