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522002111,(,515031)cDNA,,,,,Kononen,,DNAmRNA,1[1]1.1(),1.2,600m,X-Y,,X-YHE(0.6mm,3mm4mm)45mm20mm,1000,8m,,,200401HE,,3mm1mm2mm22.1p53ERBB2MucciNR[2]121002CGASYG:13%,33%,,:2001-09-07ApplicationofTissueMicroarraysforTumorMolecularPathologyTIANDong-ping,WUMing-yao:1000(FISH)FISHmRNA,DNAmRNA,:;;;:R73-3;R730.23:C:1004-0242(2002)01-0052-02200211153[1][2][3][4][5]:10%,645,10,22.2(FISH):FISH,,70%2SSC745,,CCND1,FITCERBB237,,0.2mDAPISchraml[3]17397FISHCCND1CMYCERBB23,CCND1,CMYC,3FISH,FISH397BubendorfL,FISH,FISH[4]2.3mRNA:,,ERBB2mRNA,X03363,350396,333PATP100l1107cpm/ml,4218h,1SSC55,,3,BubendorfL[5]cDNA,3(1),1000,(2)200,(3),,(4)(5),(6),,(7),(8)DNAmRNA4(1),,FISH(2),,200,DNAmRNA,(FISH)mRNA,,,::KononenJ,BubendorfL,KallioniemiA,etal.Tissuemicroar2raysforhigh2throughputmolecularprofilingoftumorspeci2ments[J].NatMed,1998,4(7):767-768.MucciNR,AkdasG,ManelyS,etal.Neuroendocrineexpres2sioninmetastaticprostatecancer:evalutionofhighthroughputtissuemicroarraystodetectheterogeneousproteinexpression[J].HumPathol,2000,31(4):406-414.SchramlP,KononenJ,BubendorfL,etal.Tissuemicroarraysforgeneamplificationsurveysinmanydifferenttumortypes[J].ClinCancerRes,1999,5(8):1966-1975.BubendorfL,KononenJ,KoivistoP,etal.Surveyofgeneam-plificationduringprosatecancerprogressionbyhigh2throughputfluorescenceinsituhybridizationontissuemicroarrays[J].CancerRes,1999,59(4):803-806.BubendorfL,KolmerM,KononenJ,etal.Hormonetherapyfailureinhumanprostatecancer:analysisbycomplementaryDNAandtissuemicroarrays[J].JNatlCancerInst,1999,91(20):1758-1764.
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本文标题：组织芯片技术在肿瘤分子病理学研究中的应用
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