您好，欢迎访问三一刀客
当前位置：首页 > 商业/管理/HR > 企业文化 > 城市生活污水和生活垃圾渗滤液抗生素抗性基因污染的比较研究黄福义
3710201610ENVIＲONMENTALSCIENCEVol．37No．10Oct．201611212121*1.3610212．100049PCＲ．39187P＜0.05119P＜0.05tnpA-04blaVEB33381061β-．PCＲX171.5A0250-3301201610-3949-06DOI10．13227/j．hjkx．2016．10．0372016-03-042016-05-162016YFD080020531270153212100081987～E-mailfyhuang@iue．ac．cn*E-mailjqsu@iue．ac．cnComparativeInvestigationofAntiboticＲesistanceGenesBetweenWastewaterandLandfillLeachateHUANGFu-yi1LIHu12ANXin-li12OUYANGWei-ying12SUJian-qiang1*1．KeyLaboratoryofUrbanEnvironmentandHealthInstituteofUrbanEnvironmentChineseAcademyofSciencesXiamen361021China2．UniversityofChineseAcademyofSciencesBeijing100049ChinaAbstractTheincreasingoutputofwastewaterandlandfillleachateduringtherapidurbanizationexacerbatedthehumanhealthrisksinducedbyantibioticresistancegenesAＲGs．ForfurtherinsightintoantibioticresistancegenesAＲGsofwastewateraddingleachatebeforetreatmentandlandfillleachatehigh-throughputqPCＲtechniquewasusedtoinvestigatetheirabundanceanddiversities．Atotalof187uniqueAＲGsweredetectedinwastewaterwhichwassignificantlyhigherthanthatinleachate39uniqueAＲGsweredetectedP＜0.05．Atotalof119antibioticresistancegenesweresignificantlyenrichedinwastewatercomparedwiththelandfillleachateP＜0.05especiallythetnpA-04andblaVEBwhichbelongstomobilegeneelementsMGEsandβ-Lactamaseincreasedby3338-foldand1061-foldrespectively．OurresultsshowedthattheprofileofAＲGswassignificantlydifferentbetweenthewastewaterandleachateandthewastewaterwastheimportantreservoirofAＲGs．Keywordswastewaterlandfillleachateantibioticantibioticresistancegeneshigh-throughputqPCＲ．．、、．12、34、5、6789．Pruden102006．、、、、、1112．、、、．“”．、375a13．14～16．1317．1819、．．、．11.1201571L350%．201571L3．－20℃DNA．1.2DNArawwastewaterＲWWrawleachateＲLC40mL50mL12000r·min－110min1mL．1.5mL18000r·min－1．FastDNASpinKitforSoilMPBiomedicalsPBS978μL．PBSLysingMatrixEtubeDNA1%．DNAQuantiFluordsDNASystemPromegaCorporationDNA．DNA50ng·μL－1．1.3PCＲSmartChipＲeal-TimePCＲSystemsWaferGenInc．．2931．1blaNDM-1201intI1class1integron211CintI1clinicalclass1integon22．PCＲ100nL．1×LightCycler480SYBＲGreenⅠMasterMixＲochenuclease-freePCＲ-gradewater1ng·μL－1BSA5ng·μL－1DNA1μmol·L－1．PCＲ95℃10min95℃30s60℃30s40．PCＲDNA．qPCＲCycler1.8～2.2．SmartChipＲeal-TimePCＲSystemsCT31．333．1.4generelativecopynumberLooft231．Schmittgen24FCfoldchange2．=1031－CT/10/31059310ΔCT=CTAＲG－CT16SΔΔCT=ΔCTTarget－ΔCTＲef2FC=2－ΔΔCTCTPCＲAＲG16S16SrDNATargetＲefFCAＲGs．FC=2－ΔΔCT+2s＞1studentt．22.1296Aminoglycoside、β-β-Lactamase、Chloramphenicol、--BMLSB、Sulfonamide、Tetracycline、Vancomycinother/efflux8．ＲWW187391．8Aminoglycoside、--BMLSB、Tetracyclineother/efflux4．62526．4．deactivation、efflux、protectionunkown．deactivation48.13%51.28%2．39erm3538．P＜0.05．1Fig．1Numberofdetectedantibioticresistancegenes2Fig．2Ｒatiosofmechanismindetectedantibioticresistancegenes2.2PCA198.8%3．．Auerbach27109copies·L－1．4P＜0.05．4AA11593373Fig．3PrinciplecomponentanalysisofAＲGsinwastewaterandlandfillleachateA2．BCDBC．“”285a13PCＲ．2.3、“”1317．．Schmittgen24genecopynumber5．others、、1.4×108copies·L－1、3.6×107copies·L－1．3944Fig．4PollutionprofileofAＲGsinwastewaterandlandfillleachate5Fig．5AbundanceofAＲGsinwastewaterandlandfillleachate1.1×105copies·L－1P＜0.05．13．7119259310P＜0.05tnpA-04、blaVEBblaOXA10-013338、106110016β-．horizontalgenetransferHGTMGEs7．1029．“”．6Fig．6EnrichedAＲGsinwastewaterWang25Wu26．．31．2．1ZhuYGJohnsonTASuJQetal．DiverseandabundantantibioticresistancegenesinChineseswinefarmsJ．ProceedingsoftheNationalAcademyofSciencesoftheUnitedStatesofAmerica201311093435-3440．2．5J．200945705-710．WuBQiaoMZhuYG．QuantificationoffivetetracyclineresistancegenesinsoilfromaswinefeedlotJ．AsianJournalofEcotoxicology200945705-710．3SuJQWeiBOuyangWYetal．AntibioticresistomeanditsassociationwithbacterialcommunitiesduringsewagesludgecompostingJ．EnvirontalScience＆Technology201549127356-7363．4ＲahubeTOMartiＲScottAetal．Impactoffertilizingwithraworanaerobicallydigestedsewagesludgeontheabundanceofantibiotic-resistantcoliformsantibioticresistancegenesandpathogenicbacteriainsoilandonvegetablesatharvestJ．AppliedandEnvironmentalMicrobiology201480226898-6907．5OuyangWYHuangFYZhaoYetal．IncreasedlevelsofantibioticresistanceinurbanstreamofJiulongjiangＲiverChinaJ．AppliedMicrobiologyandBiotechnology201599135697-5707．6．J．201435103869-3873．HuangFYLiHWeiBetal．Long-termmanureapplicationinducedshiftofdiversityandabundanceofantibioticresistancegenesinpaddysoilJ．EnvironmentalScience201435103869-3873．7XuLKOuyangWYQianYYetal．High-throughputprofilingofantibioticresistancegenesindrinkingwatertreatmentplantsanddistributionsystemsJ．EnvironmentalPollution2016213119-126．8XiCWZhangYLMarrsCFetal．PrevalenceofantibioticresistanceindrinkingwatertreatmentanddistributionsystemsJ．AppliedandEnvironmentalMicrobiology200975175714-5718．9．J．2013214481-487．SuJQHuangFYZhuYG．AntibioticresistancegenesintheenvironmentJ．BiodiversityScience2013214481-487．10PrudenAPeiＲTStorteboomHetal．AntibioticresistancegenesasemergingcontaminantsstudiesinnorthernColoradoJ．EnvironmentalScience＆Technology200640237445-7450．11．J．200420136-39．WeiDBHuHY．Safetyindexsystemofreclaimedandreused359337waterqualityJ．ChinaWater＆Wastewater200420136-39．12Mol
三一刀客所有资源均是用户自行上传分享，仅供网友学习交流，未经上传用户书面授权，请勿作他用。
扫描二维码
ylqcrystal
本文标题：城市生活污水和生活垃圾渗滤液抗生素抗性基因污染的比较研究黄福义
链接地址：https://www.111doc.com/doc-6548241 .html